
Dog Vitamin D Testing

Why this test matters
Vitamin D is involved in many important systems throughout the body, including immune regulation, skeletal health, and normal cellular function.
Low vitamin D levels have been associated with a range of chronic health conditions in dogs, including inflammatory disease, skin conditions, gastrointestinal disease, immune dysfunction, and some cancers. Vitamin D status may also be influenced by diet quality, digestive health, absorption, and overall nutritional balance.
Testing may be particularly helpful for:
- dogs with chronic inflammatory or immune conditions
- dogs with digestive or gastrointestinal disease
- dogs with skin disease or allergies
- dogs on homemade or restrictive diets
- pet parents seeking a more proactive approach to preventative health
Vitamin D supplementation should not be started blindly, as both deficiency and excess can be problematic.

Important considerations
This test measures circulating vitamin D levels at the time of sampling and should be interpreted alongside your dog’s diet, health history, clinical signs, and other laboratory findings.
Vitamin D requirements and ideal ranges in dogs are still an evolving area of veterinary research, and results should be used as part of a broader health assessment rather than in isolation.
Supplementation decisions should always be made carefully and ideally in consultation with your veterinarian.
What’s included
- Everything needed for your vet to collect and submit your dog’s blood sample
- Please note your veterinary clinic will need to centrifuge the blood sample prior to submission
- Laboratory measurement of circulating vitamin D levels using LC-MS/MS technology
- Clear result reporting
- Guidance to support nutritional and preventative health discussions